is an attempt to develop a Java-based program to reduce much of the
involved in obtaining FRET parameters for various donor/acceptor systems. It integrates many of the
equations used for doing FRET calculations with a database of
commercially available (predominantly from Invitrogen) and user defined chromophores. Currently, it's being developed as a
plugin for the InstruView
program in order to make it easier to import spectral data and reduce
programing complexity, but since it is an open-source program (Download Source Code) it
can be modified to run as a standalone program.
Download and Installation
Since FRETView is written as a plugin for InstruView, it requires the installation of this program (Download InstruView Here).
- Once the program is installed and running, please review the help manual which can be found here,or within the program. Any questions or suggestions feel free to contact Nathan (firstname.lastname@example.org).
FRETView in Action
Here are a few screenshots highliting some of the key features of the
program. The help manual provides a more detailed overview of the
Figure 1. The main program window showing the simple and
intuitive user interface. Anyone familiar with FRET
should find it relatively easy to use the program.
Figure 2. The interface used to manage the spectral database.
As can be seen, it provides a simple means to manage
database of donors and acceptors including the ability to download a
database from a webserver.
Figure 3. Plot showing the energy transfer efficiency and
intensity variation of the donor (Fd), acceptor (Fa)
intensity (Ft) as a function of donor/acceptor distance while exciting
at 490 nm.
Figure 4. Plot of the simulated spectra when the donor (Alexa
488) and acceptor (Alexa 546) are ~ 70 Å
apart and the excitation
wavelength is 490 nm. This distance can be easily varied by
moving the slider bar.
The simulation only takes into account
energy transfer so it will likely differ from experimental results if
any other photophysical effects are taking place.
Figure 5. Plot of the simulated decay profiles when a long-lived donor, Ru(phen)3,
undergoes energy transfer
with the short-lived acceptor, Cy5.
As can be seen, the lifetime of the acceptor is increased
significantly as a result of the energy transfer process. For a
D-A distance of 30 Angs and a transfer efficiency of 94% the lifetime
the Cy5 acceptor is increased to ~56ns. The lifetime of Cy5 is
normally less than two nanoseconds.